{"id":588,"date":"2021-07-27T16:16:43","date_gmt":"2021-07-27T14:16:43","guid":{"rendered":"https:\/\/blogs.ugr.es\/photochem\/?p=588"},"modified":"2021-07-27T16:16:43","modified_gmt":"2021-07-27T14:16:43","slug":"characterization-of-the-first-chloride-sensitive-rfp","status":"publish","type":"post","link":"https:\/\/blogs.ugr.es\/photochem\/characterization-of-the-first-chloride-sensitive-rfp\/","title":{"rendered":"Characterization of the first chloride-sensitive RFP!"},"content":{"rendered":"<p><span data-contrast=\"auto\">Our group has identified and improved by rational mutagenesis the first chloride-sensitive red fluorescent protein (RFP).<\/span><\/p>\n<p><span data-contrast=\"auto\">This protein presents a <\/span><i><span data-contrast=\"auto\">cis\u2013trans<\/span><\/i><span data-contrast=\"auto\"> isomerization of the chromophore that produces a dual emission band (green and red) with different chloride sensitivities, which allowed us to develop a ratiometric methodology to measure intracellular chloride concentrations. You can have a look here, DOI: <a href=\"https:\/\/pubs.acs.org\/doi\/10.1021\/acssensors.1c00094\" target=\"_blank\" rel=\"noopener\">10.1021\/acssensors.1c00094<\/a><\/span><span data-ccp-props=\"{&quot;201341983&quot;:0,&quot;335559739&quot;:160,&quot;335559740&quot;:259}\">\u00a0<\/span><\/p>\n<p><img loading=\"lazy\" decoding=\"async\" class=\"aligncenter wp-image-589 size-full\" src=\"https:\/\/blogs.ugr.es\/photochem\/wp-content\/uploads\/sites\/61\/2021\/07\/se1c00094_0007.png\" alt=\"\" width=\"500\" height=\"279\" srcset=\"https:\/\/blogs.ugr.es\/photochem\/wp-content\/uploads\/sites\/61\/2021\/07\/se1c00094_0007.png 500w, https:\/\/blogs.ugr.es\/photochem\/wp-content\/uploads\/sites\/61\/2021\/07\/se1c00094_0007-300x167.png 300w\" sizes=\"auto, (max-width: 500px) 100vw, 500px\" \/><\/p>\n<p><strong>Check out the manuscript:\u00a0<\/strong><\/p>\n<p><span data-contrast=\"auto\">New Red-Emitting Chloride-Sensitive Fluorescent Protein with Biological Uses<\/span><span data-ccp-props=\"{&quot;201341983&quot;:0,&quot;335559739&quot;:160,&quot;335559740&quot;:259}\">\u00a0.\u00a0<\/span><span data-contrast=\"auto\">Salto et al. <\/span><i><span data-contrast=\"auto\">ACS Sensors<\/span><\/i> <b><span data-contrast=\"auto\">2021<\/span><\/b> <i><span data-contrast=\"auto\">6<\/span><\/i><span data-contrast=\"auto\"> (7), 2563-2573. DOI: 10.1021\/acssensors.1c00094.<\/span><\/p>\n","protected":false},"excerpt":{"rendered":"<p>Our group has identified and improved by rational mutagenesis the first chloride-sensitive red fluorescent protein (RFP). This protein presents a cis\u2013trans isomerization of the chromophore that produces a dual emission band (green and red) with different chloride sensitivities, which allowed us to develop a ratiometric methodology to measure intracellular chloride concentrations. You can have a&hellip; <a href=\"https:\/\/blogs.ugr.es\/photochem\/characterization-of-the-first-chloride-sensitive-rfp\/\" class=\"more-link\">Leer m\u00e1s <span class=\"screen-reader-text\">acerca de Characterization of the first chloride-sensitive RFP!<\/span><\/a><\/p>\n","protected":false},"author":508,"featured_media":0,"comment_status":"open","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"_genesis_hide_title":false,"_genesis_hide_breadcrumbs":false,"_genesis_hide_singular_image":false,"_genesis_hide_footer_widgets":false,"_genesis_custom_body_class":"","_genesis_custom_post_class":"","_genesis_layout":"","footnotes":"","_members_access_role":[],"_members_access_error":""},"categories":[11],"tags":[8,9,10],"class_list":["post-588","post","type-post","status-publish","format-standard","category-papers","tag-news","tag-research","tag-tg-diag","entry","has-post-thumbnail"],"_links":{"self":[{"href":"https:\/\/blogs.ugr.es\/photochem\/wp-json\/wp\/v2\/posts\/588","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/blogs.ugr.es\/photochem\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/blogs.ugr.es\/photochem\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/blogs.ugr.es\/photochem\/wp-json\/wp\/v2\/users\/508"}],"replies":[{"embeddable":true,"href":"https:\/\/blogs.ugr.es\/photochem\/wp-json\/wp\/v2\/comments?post=588"}],"version-history":[{"count":1,"href":"https:\/\/blogs.ugr.es\/photochem\/wp-json\/wp\/v2\/posts\/588\/revisions"}],"predecessor-version":[{"id":590,"href":"https:\/\/blogs.ugr.es\/photochem\/wp-json\/wp\/v2\/posts\/588\/revisions\/590"}],"wp:attachment":[{"href":"https:\/\/blogs.ugr.es\/photochem\/wp-json\/wp\/v2\/media?parent=588"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/blogs.ugr.es\/photochem\/wp-json\/wp\/v2\/categories?post=588"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/blogs.ugr.es\/photochem\/wp-json\/wp\/v2\/tags?post=588"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}