2022 Ruiz-Arias, A., et al., A FRET pair for quantitative and superresolution imaging of amyloid fibril formation. Sensors and Actuators: B-Chemical, (2022) 350: p. 130882. DOI: 10.1016/j.snb.2021.130882. Open access. The project PID2020-114256RB-I00 is funded by … Leer más acerca de Treg-KinSens – Publications
Our recent paper, with Prof. Luis Álvarez de Cienfuegos, about the mechanism of co-assembly in short-peptide hydrogels has been selected as an HOT Article in the journal Polymer Chemistry. Check it out: Insights into the co-assemblies formed by different aromatic short-peptide amphiphiles … Leer más acerca de HOT Article in Polymer Chemistry
In our last contribution at ACS Sensors, we report an amazing methodology (DYNEX) to determine specific intracellular microenvironments taking advantage of excimer kinetics of acridones. Check it out! … Leer más acerca de Cover in ACS Sensors
Our group has identified and improved by rational mutagenesis the first chloride-sensitive red fluorescent protein (RFP). This protein presents a cis–trans isomerization of the chromophore that produces a dual emission band (green and red) with different chloride sensitivities, which allowed us … Leer más acerca de Characterization of the first chloride-sensitive RFP!
Our recent collaboration with Prof. Alvarez de Cienfuegos, studying the formation of supramolecular peptide hydrogels has been published in Materials Chemistry Frontiers. Using advanced, multiparametric fluorescence microscopy and single-molecule fluorescence spectroscopy, we have studied the … Leer más acerca de see hydrogel fibrils grow!
We start after the summer break with the release of the front cover of the September issue of Organic & Biomolecular Chemistry (vol. 18, n. 33). The manuscript, entitled N-Methyl-β-carboline alkaloids: structure-dependent photosensitizing properties and localization in subcellular domains, … Leer más acerca de Front cover in OBC
Check out our recent work on a fluorogenic sensor for N-aminopeptidase activity in bacteria, useful for two-photon and super-resolution imaging. Valverde-Pozo et al. Sensors and Actuators B (2020), 321, 128487. … Leer más acerca de Our first paper using STED microscopy
Happy to be back in the lab! Remote group meetings, and a bunch of new papers during confinement. These are some of the new papers that came out in the last couple of months. Gonzalez-Vera et al. Unusual spectroscopic and photophysical properties of solvatochromic BODIPY analogues of … Leer más acerca de Back on track!!!
Our long-lasting collaboration with DestiNA Genomica and Optoi Microelectronics, within the framework of the miRNA-DisEASY project, has resulted in an exciting paper published in Chemical Communications. In this paper we describe a new method, using DestiNA's dynamic labelling chemistry of … Leer más acerca de microRNA detection method
The combination of semi-conductor Quantum Dots (QDs) and FLIM microscopy presents countless advantages for intra-cellular imaging. With these advantages in mind, we have been working in several nanosenors in the last few years. Our latest work on intracellular nanosensors, specifically designed … Leer más acerca de A couple of sensors
In previous works, we used the fluorescent dye 2-Me-4-OMe-TM as intracellular phosphate sensing through fluorescence lifetime imaging microscopy (FLIM). However, a lower sensitivity than expected was observed. The use of fluorescent dyes needs the understanding of their photophysical properties … Leer más acerca de Unraveling a silicon-substituted xanthene
In a fruitful collaboration with Prof. Peter Friedman, from Pittsburgh University, we have provided insights into the mechanisms of NHERF1 phosphorylation promoted by parathyroid hormone, regulating NPT2A-dependent phosphate transport. We have elegantly used our FLIM-sensor to follow transient … Leer más acerca de Insights into NPT2A-dependent phosphate transport