Happy to be back in the lab! Remote group meetings, and a bunch of new papers during confinement. These are some of the new papers that came out in the last couple of months. Gonzalez-Vera et al. Unusual spectroscopic and photophysical properties of solvatochromic BODIPY analogues of Prodan. Dyes Pigments 2020, DOI: 10.1016/j.dyepig.2020.108510. Fueyo-Gonzalez et … [Leer más...] acerca deBack on track!!!
Our long-lasting collaboration with DestiNA Genomica and Optoi Microelectronics, within the framework of the miRNA-DisEASY project, has resulted in an exciting paper published in Chemical Communications. In this paper we describe a new method, using DestiNA's dynamic labelling chemistry of nucleic acids and based on time-gated imaging, to detect abnormal levels in … [Leer más...] acerca demicroRNA detection method
The combination of semi-conductor Quantum Dots (QDs) and FLIM microscopy presents countless advantages for intra-cellular imaging. With these advantages in mind, we have been working in several nanosenors in the last few years. Our latest work on intracellular nanosensors, specifically designed for FLIM microscopy, targeting glucose is now published in the journal Sensors … [Leer más...] acerca deA couple of sensors
In previous works, we used the fluorescent dye 2-Me-4-OMe-TM as intracellular phosphate sensing through fluorescence lifetime imaging microscopy (FLIM). However, a lower sensitivity than expected was observed. The use of fluorescent dyes needs the understanding of their photophysical properties for a correct interpretation of their signal. Because cytoplasm is a complex … [Leer más...] acerca deUnraveling a silicon-substituted xanthene
In a fruitful collaboration with Prof. Peter Friedman, from Pittsburgh University, we have provided insights into the mechanisms of NHERF1 phosphorylation promoted by parathyroid hormone, regulating NPT2A-dependent phosphate transport. We have elegantly used our FLIM-sensor to follow transient phosphate kinetics within live cells upon the interaction with PTH hormone. We … [Leer más...] acerca deInsights into NPT2A-dependent phosphate transport